Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
December, Special Anniversary  Review Issue. 2018 Vol. 7(12): pp. 377-382
Copyright © 2018 Global Advanced Research Journals

Full Length Research Paper

Antifungal activity of silver@silica nanoparticles against aflatoxigenic Aspergillus flavus

Ameth Diagne¹, Bocar Noel Diop², Lat SoukTounkara³, Caroline Andreazza⁴, Mbacké Sembène¹.

¹Département de Biologie Animale, Faculté des sciences et Techniques, Université Cheikh Anta Diop (UCAD), Senegal;

²bnd sciences, Senegal;

³Institut Technologie Alimentaire (ITA), Senegal;

⁴Interfaces, Confinement, Matériaux et Nanostructures (ICMN) - UMR 7374 CNRS - Université d’Orléans, France.

*Corresponding Author's Email: ameth.diagne@ucad.edu.sn

Accepted 3 December, 2018

Abstract

In Senegal, Aspergillus flavus, which develops on crops by producing aflatoxins, seriously affects the economy and the health of consumers. In this study, the antifungal effect of silver@silica on aflatoxigenic A. flavus was investigated. An aflatoxigenic A. flavus strain was isolated on Potato Dextrose Agar (PDA) medium from infested groundnut seeds. silver@silica nanoparticles were synthesized by reverse microemulsion and different concentrations of them were tested on A. flavus. The bioactivity type of silver@silica nanoparticles was also determined. The obtained results revealed that strong inhibition of A. flavus was noticed at all concentrations. Additionally, the bioactivity tests performed showed that silver@silica nanoparticles had a fungicide effect on A. flavus. Thus, silver@silica nanoparticles could be used as effective, safe and ecofriendly antifungals to prevent fungal growth and subsequent aflatoxins production.

Keywords: Aflatoxigenic Aspergillus flavus, antifungal activity, silver@silica nanoparticles, reverse microemulsion

REFERENCES

Acharya D, Mohanta B, Pandey P, Nasiri F (2018). Antibacterial Properties of Synthesized Ag and Ag@SiO2 Core-Shell Nanoparticles: A Comparative Study (2018). Can J Phys.96: 955-960.

Bergera TJ, Spadaro JA, Bierman R, Chapin SE, Becker RO (1976). Antifungal Properties of Electrically Generated Metallic Ions.Antimicrob Agents Chemother. 5:856-860.

Clavel D, da Sylva A, Ndoye O, Mayeux A (2013). Amélioration de la qualité sanitaire de l'arachide au Sénégal : un challenge pour une opération de recherche-développement participative. Cah Agric. 22:174-81

Egger S, Lehmann RP, Height MJ, Loessner MJ, Schuppler M (2009). Antimicrobial Properties of a NovelSilver-Silica Nanocomposite Material. Appl Environ Microbiol.75:2973–2976.

Elechiguerra JL, Burt JL, Morones JR (2005). Interaction of silver nanoparticles with HIV-1. J Nanotechnol.3:1-10.

IARC(International Agency for Research on Cancer) (1993). Some Naturally Occurring Substances: Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins.IARC.1-599pp.

Ouattara-Sourabie PB, Nikiema PA, Traore AS (2011). Caractérisation de souches d’Aspergillus sppisolées des graines d’arachides cultivées au Burkina Faso, Afrique de l’Ouest. Int J Biol. Chem Sci. 5:1232-1249.

PACA (Partnership for Aflatoxin Control in Africa) (2012). Impacts de l'aflatoxine et solutions potentielles dans les domaines de l'agriculture, du commerce et de la santé. PACA.1-13pp.

Petica A, Gavriliu S, Lungu M, Buruntea N, Panzaru C (2008). Colloidal silver solutions with antimicrobial properties. Mater Sci Eng. 152:22–27

Salem HF, KAM E, Sharaf MA (2011). Formulation and evaluation of silver nanoparticles as antibacterial and antifungal agents with a minimal cytotoxic effect. Int J Nov Drug Deliv.3:293-304.

Shehu K, Bello MT (2011). Effect of Environmental Factors on the Growth of Aspergillus Species Associated with Stored Millet Grains in Sokoto. Niger. J. Basic Appl. Sci. 19:218-223.

Voisin A (2012). Elaboration et Etude de Nouveaux Biocides Supportés Respectueux de l’environnement. Thèse de doctorat de 3ème Cyclede Polymères, Université Bordeaux 1. 1-222pp.

Zheng LP, Zhang Z, Zhang B, Wang JW (2012). Antifungal properties of Ag-SiO2 core-shell nanoparticles against phytopathogenic  fungi. Adv Mater Res. 476:814-818.