Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
November 2017 Vol. 6(11): pp. 374-380
Copyright © 2017 Global Advanced Research Journals

Full Length Research Paper

Serum immunoglobulins (IgA, IgM, IgE) and acetylcholinesterase level determination in agricultural spray workers exposed to organophosphates pesticides in district Gujranwala

Saffora Riaz¹, Farkhanda Manzoor¹, Nasir Mahmood², Ali Hassan³, Hafsa Memona¹

¹ Department of Zoology, Lahore College for Women University, Lahore, Pakistan.

² Department of Allied Health Sciences and Chemical Pathology; Department of Human Genetics and Molecular Biology, University of Health sciences, Lahore, Pakistan.

³Department of community medicine, CMH medical college, Lahore, Pakistan

Email: safforariaz@yahoo.com

Accepted 01 November, 2017

Abstract

Background: Acetylcholinesterase is enzyme instantly performs the hydrolytic cleavage of acetylcholine which is a neurotransmitter. Enzymatic degradation of neurotransmitter was inhibited by organophosphates exposure and in activation or decrease levels of acetylcholinesterase leads to accumulation of excessive acetylcholine which resulted in paralysis, respiratory failure and even death. Methods:Organophosphate impact on serum immunoglobulins (IgA, IgM and IgE) and acetylcholinesterase level was determined in pesticides exposed local agricultural spray workers (n= 200) and health control group of pesticides unexposed persons (n= 100). Results: Acetylcholinesterase level mean value in male spray workers (0.16 U/ mL; -0.10-0.66 U/ mL) and non-spray male workers (0.44 U/ mL; 0.01-0.93 U/ mL) was highly significant at p>0.05.  Similarly, highly significant difference at p>0.05 was observed in acetylcholinesterase level mean value of female spray workers (0.41 U/ mL; 0.06-0.93 U/ mL) and non-spray female workers (0.65 U/ mL; 0.32-1.1 U/ mL). Conclusion:  In current research it was observed that serum acetylcholinesterase level was reduced in the male and female spray workers as compared to non-spray workers. Mean high levels of IgA, IgM and IgE immunoglobulins were observed in both male and female spray workers. Hence it is concluded that organophosphates exert toxic impact on human health. So use of these pesticides must be minimized.

Keywords:  Acetylcholinesterase, Immunoglobulins, Organophosphate pesticides, ELISA