Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
October 2017 Vol. 6(10): pp. 310-313
Copyright © 2017 Global Advanced Research Journals

 

Full Length Research Paper

A new Rhabdo virus Infection in Maize Plantations in Turkey

Filiz  Ertunc

 

Ankara University, Faculty of Agriculture, Department of Plant Protection, 06110 Ankara,Turkey

Email: ertunc@agri.ankara.edu.tr

Accepted 05 October, 2017

 

Abstract

Maize mosaicrhabdo virus (MMV) is devastating virus infection, causes  serious crop losses, on the family Poaceae is especially on maize and transmitedby leaf  hopper, Peregrinusmaidis. Samples were collected from  leaves  and stems of maize plants, from Bartın, Düzce, Sakarya and Zonguldak provinces of Turkey, in  2012-2013. They were tested against MMV antiserum  by DAS-ELISA and  also RT-PCR assay with a primers specific to glycoprotein gene of the virus, designed in our laboratory. 15 of the  collected maize samples  were found to be infected with MMV. Positive  samples  were mechanically inoculated  to maize  plant  lets for propagation and maintain. MMV inoculated maize plants, show light mosaic symptoms were tested by ELISA and RT-PCR again, obtained bands were  470bp long. In electron microscopic observations, rhabdovirus-like particules were detected.So far to our knowledge, it is the first report of Maize mosaic rhabdovirus presence in Turkey.

Keywords:  Maize, MMV, Rhabdovirus, RT-PCR, electromicrocopy. 

 

 

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