Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
February 2016 Issue Vol. 5(2), pp. 042-052
Copyright © 2016 Global Advanced Research Journals

Full Length Research Paper

Optimized HILIC-MS/MS Method for Simultaneous Determination of two Antiepileptic Drugs with Significant Difference in Binding to Serum Albumin

Hassan A. Alhazmi^1*, Mohammed Al Bratty¹, Gunasekar Manoharan¹, Hafiz A. Makeen² and Adnan A. Kadi³

¹Jazan University, College of pharmacy, Department of Pharmaceutical Chemistry, P.O. Box 114, Jazan 45142, Saudi Arabia.
²Jazan University, College of pharmacy, Department of Clinical Pharmacy, P.O. Box 114, Jazan 45142, Saudi Arabia.
³King Saud University, College of pharmacy, Department of Pharmaceutical Chemistry, P.O. Box 2457, Riyadh 11451, Saudi Arabia.

*Corresponding Author E-Mail: hasalhazmi@gmail.com; Phone: +966 541433344

Accepted 04 February, 2016

Abstract

Different HILIC columns were studied and compared. Each column showed different behaviors regarding to the separation of four antiepileptic drugs and the influence on MS/MS sensitivity through the ionization of the drugs. HILIC Luna was selected since it has been offered good separation and sensitivity. Additionally, a fast, sensitive and selective HILIC-MS/MS method was developed for the detection and quantification of two antiepileptic drugs, carbamazepine (highly bind to serum albumin) and vigabatrin (not significant bind to serum albumin) in human plasma. Samples were purified by two steps, precipitation by methanol and using the supernatant for vigabatrin and liquid–liquid extraction using ethylacetate for carbamazepine. The separation was successfully achieved on a HILIC LUNA 3µm (2× 150 mm) column with a mobile phase consisting acetonitril/ammonium formate (5mM, pH 3.5) (75:25, v/v). A triple quadrupole model G6410A mass spectrometerin the MRM mode was used for detection using electro spray ionisation (ESI). The detections were based on the transition of the protonated molecular ion for carbamazepine at m/z 237 and vigabatrin at m/z 130 to the predominant ions of m/z 194 and 71, respectively. The acceptable mean recoveries were 81% for carbamazepine and 100% for vigabatrin. Furthermore, the calibration curves were linear over the concentration range 250–8000 ng/mL for carbamazepine and 15–960 ng/ml for Vigabatrin. The LODs for carbamazepine and vigabatrin in human plasma were 5.43 ng/mL and 3 ng/mL, respectively.

Keywords: HILIC, MS/MS, Carbamazepine, Vigabatrin.