Bio-Suppression of Strawberry Fruit Rot Disease Caused by Botrytis cinerea

Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
August 2015 Vol. 4(8): pp. 404-414
Copyright © 2015 Global Advanced Research Journals

Full Length Research Paper

Bio-Suppression of Strawberry Fruit Rot Disease Caused by Botrytis cinerea

Abeer, A. El-ghanam¹; Safinaz, A. Farfour² and Seham, S. Ragab¹

1-Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.

2-Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.

*Corresponding Author's Email: farfour.safinaz@yahoo.com

Accepted 07 August, 2015

Abstract

In grade to obtain high quality and safety food along with the environment protection from pesticides hazards, four bio agents were used to control fruit rot disease caused by Botrytis cinerea in strawberry fruits. Chlorella vulgris, Spirulina platensis, Azotobacter chroococcum, Trichoderma harizianum and their combinations (T₁, T₂, T₃, T₄,.. ..,T₁₂) were used as spray treatment in the open field and on strawberry after picking. The individual bio agents treatments decreased liner growth and spore production of B. cinerea in comparing with mixed bio agents. Also, all the treatments decreased Disease Severity % (DS%). The treatments ofT₁, T_2, T₃andT₄ caused 13.9, 22.3, 22.3 and 24.1 DS% during 2014;12.26, 15.5, 17.0 and 21.86 DS% during 2015in comparing with the mixed bio agents (T₁₂) which caused 29.3 and 29.03 DS% during 2014 and 2015, respect., after three sprayings in the field. Also, the treatments ofT₁, T_2, T₃and T₄ caused 0.00 DS% after 2^nd spray through the storage at 5˚C. The treatment of T₁₂caused the highest increase in the total sugar content in strawberry fruits which infected naturally with B. cinerea and stored at 5^◦C for three weeks increased with T_12.In addition, the treatment of T₁₂ caused the highest increase in polyphenoloxidase (PPO) activity and peroxidase (PO) which were 0.459 and 0.360U/mg fresh weight respect., in comparing with T₁, T_2, T₃and T₄ which caused 0.278, 0.287, 0.298, 0.313 and 0.139, 0.202, 0.276, 0.302 U/mg fresh weight respect., after storage for 6 weeks. The treatment of T₁ gave the best result in K% in leaves (1.82). T₅ gave the best value of N% in soil (72.2%), T3 gave the best one in P% in soil (36.0%) and T₄ caused the best amount of K% (16.0) in the soil.